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For TUNEL evaluation, an Ex filter of 365/28 nm, a DM of 405 nm, and an Em filter of 445/50 nm were used

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The procedure was performed according to the requirements of the manual test kit

After derivatization with 10 l of 5 mM 7-diethylamino-3-(4-maleimidophenyl)-4-methylcoumarin (Sigma-Aldrich) at room temperature for 10 min, the maleimide ring was cleaved by addition of 30 l of 50 mM ammonium formate (Sigma-Aldrich) pH 4.2 at room temperature for another 30 min