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Generally, hydrophobic residues form essential contacts during protein-protein interactions, whereas acidic residues mediate long-range interactions with positively charged residues 50-53

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doi:10.1016/j.peptides.2019.170202 18

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Zilbauer, E.J., Van der Goot, H.A., Bruington, A.E., Ingram, A.A., Report to the California State Water Resources Board on Investigational Work for Prevention and Control of Sea Water Intrusion