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Plates were washed twice with ice-cold PBS, scraped, pelleted 250 g at 4 C for 3 min and treated for 5 min with 500 l of cytoplasmic lysis buffer (10 mM Tris-Cl, pH 8, 0.32 M Sucrose, 3 mM CaCl 2 , 2 mM MgCl 2 , 0.1 mM EDTA, 0.5 % TritonX-100) supplemented with 20 U/ml SUPERase inhibitor (Thermo Scientific, AM2694)