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Based on existing literature, an N of 6 was initially tested for DMRS in both groups and the fMRI group of GLP-1R KO mice, an N of 7 for PET in both groups [30,31,32,33], and an N of 6 for both groups for cyclic adenosine monophosphate (cAMP) measurement

And freezing, even briefly, causes irreversible damage to the peptide structure