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On the other hand, in the presence of NADPH there was an increase in the T m of 3.57.2 C providing further evidence that the ES complex is the target of the inhibitors

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BioRad, Hercules, CA, USA) and real-time quantitative PCR (qPCR) was performed using a TaqMan 7900 Sequence Detection System with TaqMan Universal PCR Master Mix and TaqMan Gene Expression Assays (Applied Biosystems, Foster City, CA, USA)

doi: 10.1186/s12916-016-0693-4 77 De Fatima dos Santos TeixeiraFBorges dos SantosPCabanelas Pazos-MouraC