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Excess secondary antibody was washed off by 3 consecutive 10-min rinses with PBST

Physically, chronic alcohol use can lead to severe health problems, such as liver cirrhosis, alcoholic cardiomyopathy, and an increased risk of various cancers

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doi: 10.1016/j.freeradbiomed.2011.04.010

Multiple cellular processes are considered to contribute to this functional decline and these are collectively referred to as the hallmarks of aging and these include processes like genomic instability, telomere attrition, epigenetic alterations, loss of proteostasis, disabled macroautophagy, deregulated nutrient-sensing, mitochondrial dysfunction, cellular senescence, stem cell exhaustion, altered intercellular communication, chronic inflammation, and dysbiosis 1