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167,168 In hepatocytes, elevated lipid load and insulin resistance increase mitochondrial -oxidation activity, accompanied by electron leakage and respiratory chain disruption, resulting in substantial ROS production, which exacerbates lipid peroxidation, autophagic dysfunction, and pyroptosis

Then, the membranes were incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies (1:20000, Jackson ImmunoResearch, West Grove, PA) for 1 h at room temperature

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The discomfort level is minimal, comparable to the insulin injections that millions of diabetic Canadians perform daily