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For serum preparation, blood samples were incubated for 1 h at room temperature and then centrifuged (1,950 x g, 15 min, Varifuge 3.0, Heraeus, Hanau, Germany) and stored at -80C prior to further analysis

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Studies indicate that individuals treated with Tirzepatide typically lose an average of 15% to over 20% of their initial body weight, depending on dosage, duration of treatment, baseline health, and other individual factors