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The eluted protein was concentrated using an Amicon 100 kDa MWCO Ultra spin column (Millipore) and then subjected to size-exclusion chromatography using a Superose 6 increase 10/300 GL column (Cytiva) equilibrated with 1xTBS, 0.02% GDN

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Groups at higher risk of deficiency include older adults (due to reduced stomach acid production), vegans and vegetarians, people with pernicious anaemia or gastrointestinal disorders affecting absorption, and those taking certain medications such as metformin or proton pump inhibitors

While there are many factors involved in the pathogenesis of these diseases, several studies from different laboratories have demonstrated that catalase has a relationship with the pathogenesis of these diseases