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Water was offered ad libitum in two nipple drinkers per cage and feed intake was restricted to 115 g/d/hen in linear feeders

Statistical significance was assessed by ordinary One-Way ANOVA followed by Dunnetts multiple comparisons test in b , c , d

Shaking introduces strong shearing forces that can physically break the fragile peptide bonds

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Supplementation of culture medium with DHA causes a nearly immediate incorporation by deacylation-reacylation mechanisms, whereby some monounsaturated fatty acids (mainly oleic acid) are readily replaced with DHA in membrane phospholipids (Farooqui et al., 2006)