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Establishment of diabetic mice Following an overnight fast, mice were injected intraperitoneally with streptozotocin (STZ) at a dose of 150 mg/kg to induce T2DM [27], while control mice received an equivalent volume of sodium citrate buffer

Surgery and Procedure for Intracerebroventricular (ICV) Injections Rats were implanted with a permanent 22-gage guide cannula (Plastics One, Bilaney, Germany) placed into the lateral ventricle (coordinates 0.8 mm posterior from bregma, 1.4 mm lateral from midline, 5.0 mm below the surface of the brain)

Always gently swirl or roll the vial to dissolve the powder

Bile acid absorption in the jejunum is a passive, non-saturable and concentration-dependent process, whereas ileal absorption is mainly an active process [73]

Zepbound and Mounjaro are Eli Lilly's name brands of tirzepatide