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Gaetanoli TR, Argenziano G

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After reconstitution, refrigerate at 28 C, avoid repeated freezethaw cycles, and use within laboratorydefined timeframes

Results Inhibition of glutathione metabolism depletes neuronal glutamate and affects excitatory transmission To test the hypothesis that glutathione is a significant reservoir for glutamate, we treated neuronal cells with molecular inhibitors targeting enzymes of the glutathione metabolic cycle: L-2-imidazolidone-4-carboxylate (2I4C), acivicin, L-buthionine sulfoximine (BSO), and sulforaphane