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In brief, luciferase-labeled 4T1 tumor cells (20 4 cells/mice) or E0771 tumor cells (0.5 10 6 cells/mice) were resuspended in 50:50 solutions of PBS and Matrigel (Corning) and injected into the fourth mammary fat pad of 56-week-old BALB/c or C57BL/6 mice